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KMID : 0368420160590020114
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Journal of Plant Biology
2016 Volume.59 No. 2 p.114 ~ p.120
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Embryological and genetic evidence of amphimixis and apomixis in Boehmeria tricuspis
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Tang Qing
Zang Gonggu
Zhao Lining
Cheng Chaohua
Dong Zhixue
Gao Chunsheng
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Abstract
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Apomixis is a widespread alternative mode of sexual reproduction resulting in offspring that are genetically identical to the maternal plant. Boehmeria tricuspis (Hance) Makino is a perennial, wind-pollinated, herbaceous plant in the nettle family Urticaceae. The diploid B. tricuspis is monoecious but the triploid B. tricuspis is gynoecious, bearing female inflorescences only. Apomixis in B. tricuspis was first reported 50 years ago, but the mode of apomixis in the species has not been described yet. Here, we provide embryological observations of the embryo sac formation proving that triploid B. tricuspis reproduced apomictically following the Antennaria type of diplospory, and that the diploid individuals were the sexual genotype with the classical Polygonum-type maturation pattern of embryo sac development. A subsequent flow cytometry seed screen (FCSS) showed that the triploids were obligate apomicts with autonomous endosperm development, and the diploids reproduced sexually. In addition, a progeny test by molecular marker assays further demonstrated the above results.
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KEYWORD
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Apomixis, Boehmeria tricuspis, Diplospory, Flow cytometry seed screen
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